Anti-inflammatory and Antioxidant Activity of Solenostemma argel extract

The potency of Solenostemma argel (asclepiadaceae) as anti-inflammatory and antioxidant effects was investigated. Three solvent extract were used for extracting (polyphenolic, flavonoids and tannins) from the leaves of Solenostemma argel (S. argel). Three complementary antioxidant systems: (DPPH∙ free radical scavenging, Fe2+-chelating and iron reducing power) as well as the total phenolic, total flavonoids and total tannins contents of the extracts was also investigated. In-vitro anti-inflammatory effects of S. argel were assessed by using albumin denaturation assay. Our findings indicated that S. argel acetone extract exhibited greater scavenging activity than those extracted by other solvents. The same trend was achieved by using Fe2+-chelating and reducing power. The results revealed that the aqueous, acetone and ethanol extracts of S. argel showed anti-inflammatory activity (74.19%, 69.44% and 66.58%) respectively by inhibiting the heat induced albumin denaturation. The outcomes from HPLC study showed that acetone extract from S. argel had the highest amounts of e-vanillic, benzoic, pyrogallol, chlorogenic and ellagic acids. Whereas, rosmarinic, quercetrin and narengin acids were the major flavonoids compounds in S. argel acetone extract. The obtained results showed that, the solvent play a vital role in the extraction of the plant constituents and S. argel is a very promising source of bioactive compounds. Therefore, it could be concluded that flavonoids and related polyphenols present in the S .argel extract may be responsible for the anti-inflammatory and antioxidant activities which may be attributed to the radical scavenging and anti-inflammatory effects of some of its active constituents.